The overwhelming majority of non-denominational evangelical churches hold Sunday as the primary day for corporate worship, grounded in Christ's resurrection on the first day of the week and the early church's practice of meeting on Sunday. This is not typically framed as strict Sabbatarianism — the application of the Fourth Commandment's specific regulations to Sunday — but as an appropriate Christian observance of the Lord's Day.

Most non-denominational evangelical theologians hold that the Mosaic Sabbath commandment's ceremonial aspects have been fulfilled in Christ (Colossians 2:16-17) and that Christians are not bound by its specific regulations. The principle of one day in seven set apart for rest and worship is often affirmed as a creation ordinance with enduring relevance, but the legalistic enforcement of Sabbath rules is not.

In practice, non-denominational evangelical culture does not typically restrict Sunday activities beyond attending church. Sports, recreation, work, and entertainment on Sundays are not treated as sin. Individual families and believers exercise personal discernment about how to honor the Lord's Day, consistent with the broader evangelical emphasis on liberty of conscience in matters not clearly defined by Scripture.