Southern Baptists generally observe Sunday as the Lord's Day — the day of Christ's resurrection — as the primary day for corporate worship and Christian gathering. However, this is not framed as the observance of the Jewish Sabbath commandment transferred to Sunday. Most SBC theologians hold that the ceremonial aspects of the Mosaic Law, including the specific Sabbath regulations, have been fulfilled in Christ and are not binding on Christians in the same way.

The BFM2000 does not directly address Sabbath observance in detail. Sunday is treated practically as a day of worship and rest in most Southern Baptist contexts, but its basis is understood as the resurrection of Christ (the 'first day of the week') rather than the application of the Fourth Commandment. SBC churches typically gather on Sunday for morning and sometimes evening services.

Some Southern Baptists, influenced by Reformed theology, hold a stricter Lord's Day view approaching Sabbatarianism. Most SBC churches, however, do not mandate abstention from work or recreation on Sundays and do not treat Sunday observance as a mark of faithfulness comparable to Seventh-day Adventist Sabbatarianism.