The Plymouth Brethren affirm the traditional Trinitarian reading of John 1:1, interpreting the opening declaration that "the Word was God" as a clear affirmation of Christ's eternal deity and full divinity. This interpretation aligns with the Apostolic Fathers and medieval theological consensus, viewing the Word (Logos) as coeternal and coequal with God the Father. The passage establishes Christ's divine nature prior to and independent of the Incarnation, making it foundational to Brethren Christology.

Brothers like J.N. Darby extensively commented on this passage in their biblical expositions, emphasizing that John 1:1-3 demonstrates Christ's participation in creation as the eternal Word of God. The text's doctrinal weight in Trinitarian theology made it a reference point in Brethren catechesis and preaching. For the Brethren, John's Prologue establishes both the personal distinction of the Word and the full identity with the divine nature, essential to their affirmation of classical Trinitarian doctrine.