Mennonites affirm the Holy Spirit as the third person of the Trinity, active in the life of the church and the individual believer. The Spirit convicts, regenerates, sanctifies, and empowers believers for witness and service. The Spirit also guides the community in discerning God's will through Scripture, prayer, and corporate decision-making.

Mennonites are neither Pentecostal nor cessationist in the strict sense. The Spirit's ongoing guidance is affirmed, but dramatic charismatic manifestations have generally not been a major feature of mainstream Mennonite spirituality. The Spirit's work is associated with the transformation of character toward the image of Christ and the empowerment for peaceful witness.