Churches of Christ worship on Sunday as the Lord's Day, in commemoration of Christ's resurrection and consistent with the New Testament pattern of the first day of the week (Acts 20:7, 1 Corinthians 16:2). The Mosaic Sabbath law is not binding on New Testament Christians; it was part of the old covenant that has been fulfilled and superseded in Christ.

Sabbatarian movements are rejected as misapplying the Old Testament law to the Christian era. Churches of Christ take seriously the New Testament authorization of Sunday as the day of assembly and the Lord's Supper, treating this pattern as binding. There is, however, no teaching that rest or worship on Saturday is sinful — only that Saturday Sabbath-keeping is not a New Testament requirement.