Vineyard holds that the Saturday Sabbath of the Mosaic law has been fulfilled in Christ and is not binding on New Testament believers in its original form. Sunday is observed as the Lord's Day in commemoration of the Resurrection, and regular corporate worship and rest are strongly encouraged as spiritual disciplines.

Vineyard does not treat the Sabbath question as a significant doctrinal issue. The movement's focus is on Kingdom life — worship, community, mission, and ministry — with Sunday gatherings as the central communal expression. The principle of regular rest and worship is affirmed, but the specific day is treated as a matter of Christian freedom.