The True Jesus Church distinctively teaches that the Saturday Sabbath (the seventh day) is binding on all Christians and is one of the five sacramental requirements for salvation. This is a unique position among Pentecostal churches and aligns the True Jesus Church with Seventh-day Sabbatarian movements. The Sabbath is not merely recommended but is commanded by God and must be observed from Friday evening to Saturday evening (sundown to sundown).

The church grounds this requirement in the Ten Commandments (Exodus 20:8-11) and teaches that the Sabbath was never repealed by Christ or the apostles. The supposed Christian shift to Sunday worship is seen as a human tradition contrary to God's law. Proper Sabbath observance involves rest from work, gathering for worship and communion, and spiritual focus on God.

The Sabbath is elevated to sacramental status alongside water baptism and the other four requirements, making it inseparable from salvation. This distinctive emphasis is rooted in the church's belief that it has restored the original apostolic faith, which maintained Sabbath observance. The practice is particularly challenging for members in industrialized Western societies and has been a significant factor in the church's growth in societies where Sabbath observance is more culturally accommodated.