The Plymouth Brethren hold a nuanced position on Genesis 1:1-2, affirming that God is the Creator of all things while permitting theological diversity on the mechanism and age of creation. Some Brethren embrace a literal six-day creation with a young earth; others accept an old-earth view, interpreting the "days" of Genesis 1 as symbolic or geological ages. Still others explore theistic evolution as compatible with God's creative sovereignty. This flexibility reflects the Brethren's principle of biblical fidelity without unnecessary dogmatism on matters not explicitly defined by Scripture.

The core Brethren conviction is that God's special creation of humanity in his image (Genesis 1:27) and his transcendent design of the created order are non-negotiable, but the precise mechanism by which God accomplished creation remains open to scholarly investigation and hermeneutical disagreement. J.N. Darby himself offered typological and symbolic readings of Genesis that opened interpretive space for various scientific frameworks. What matters doctrinally is God's purposeful authorship and humanity's distinct place as image-bearers; the age of the earth and mode of biological development are secondary concerns on which faithful Brethren believers may differ in good conscience.