Disciples affirm the Holy Spirit as the third person of the Trinity, active in the life of the church and the believer. The Spirit guides the community into truth, empowers witness, and brings unity. Disciples are not Pentecostal and do not have a tradition of tongues or healing gifts, but they affirm the Spirit's ongoing presence and work.

The denomination's ecumenical orientation means that charismatic expressions are not foreign to some Disciples congregations, but they are not normative. The Spirit is typically associated with guidance in scripture interpretation, calls to justice, and the unity of the church rather than with dramatic miraculous gifts.