The Church of the Nazarene worships on Sunday as the Lord's Day, the day of Christ's resurrection, following the practice of the early church. Sunday worship is considered the appropriate Christian observance of the Sabbath principle, though the denomination does not impose the detailed legal restrictions of the Old Testament Sabbath commandment on Sunday practice.

The Wesleyan tradition has generally held that the Sabbath principle — one day in seven set apart for rest and worship — is a creation ordinance with enduring relevance, but that the specific ceremonial regulations of the Mosaic Sabbath were part of the typological law fulfilled in Christ. Christians observe the Lord's Day as a day of worship, rest, and renewal, but the specific legal restrictions (no work, no travel) are not binding in the same way.

The Nazarene Covenant of Christian Conduct addresses many practical lifestyle questions, but Sabbath observance per se is not a formal issue of church discipline. Individual families and members are encouraged to honor Sunday as a day of rest and worship, but the denomination does not mandate specific rules about Sunday activities beyond participation in the corporate worship life of the church.