Calvary Chapel affirms the virginal conception and birth of Jesus as a literal, historical miracle. Mary conceived by the power of the Holy Spirit without any human father, fulfilling the prophecy of Isaiah 7:14. This doctrine is considered essential to a proper understanding of the Incarnation and is a non-negotiable element of orthodox Christianity as Calvary Chapel understands it.

The virgin birth is regularly taught in Calvary Chapel churches, particularly during Advent, as a demonstration of both Christ's divine origin and his genuine humanity born of a human mother.