The Brethren in Christ observe Sunday (the Lord's Day) as the primary day of worship, following Christian tradition. Sunday commemorates Christ's resurrection and is the day of congregational gathering, prayer, and celebration.

The Sabbath principle (a day of rest and holy time) is affirmed, but it is not rigidly tied to Saturday or to Old Testament Sabbath laws. The Brethren in Christ do not enforce strict Sabbath observance (work prohibitions, travel restrictions, etc.) in the manner of Seventh-day Adventists or Orthodox Judaism.

Rather, the underlying principle—that God sanctifies time, that rest and worship are important, that families should gather for fellowship—is affirmed. Individual believers may set aside time for rest and reflection, but this is not mandated as a law.